Protective effects of Heat-killed <i>Lactobacillus sakei</i>CVL-001 on DSS-induced ulcerative colitis in mice

Abstract Recently, the incidence of Inflammatory Bowel Disease (IBD) is increasing due to westernized lifestyle and eating habits. IBD a representative gastrointestinal immune disease, casued by an excessive response in tract. In recent studies, lactic acid bacteria which known as beneficial intestinal microbiota have protective effects on various mechanisms. Here, we evaluated effect Lactobacillus sakei CVL-001 isolated from korean tradtional food kimchi against dextran sulfate sodium-induced colitis mice. First, administration Heat-killed (HK) showed DSS-induced colitis. Also, colonic mRNA expression levels that pro-inflammatory cytokine was downlregulated anti-inflammatory upregulated. Nucleotide-oligomerization domain 2 (NOD2) well innate receptor deeply related IBD. We found HK induced NOD2-dependent regulatory phenotype mouse bone marrow derived dendritic cell (BMDCs). Especially, demonstrated CD103, marker tolerogenic DC, expressed mediated NOD2. vivo colon administered with favoured expansion mucosal CD103 DCs T cells. However, abolished NOD2 knockout This results indicated Heat killed L. might be used therapeutic agent strategy research supported Mid-Career Researcher Programs (Grant No. 2022R1A2C2012287) National Research Foundation Korea funded Ministry Science ICT (Information Communication Technologies).